Journal: International Journal of Molecular Sciences

Article Title: ELTD1 Activation Induces an Endothelial-EMT Transition to a Myofibroblast Phenotype

doi: 10.3390/ijms222011293

Figure Lengend Snippet: Pro-angiogenic secreted factors are released by coELTD1 expressing cells, including IL1. ( A ) ProteomeProfiler TM Angiogenesis Array (R&D Systems) using supernatant collected from control and coELTD1 HUVEC collected at 120 h. ( B ) Quantification of the Angiogenesis array and comparison with RNA expression level. ( C ) Quantification of IL1 and IL8 secretion at 120 h using ELISA. ( D ) Expression of IL1 target genes upon IL1 treatment +/− IL1-receptor antagonist. * p < 0.05; ** p < 0.005; *** p < 0.0005.

Article Snippet: IL1-receptor antagonist (Sigma, St. Louis, MO, USA) was added at 1 μg/mL.

Techniques: Expressing, RNA Expression, Enzyme-linked Immunosorbent Assay

Journal: Cells

Article Title: Methylglyoxal Acts as a Tumor-Promoting Factor in Anaplastic Thyroid Cancer

doi: 10.3390/cells8060547

Figure Lengend Snippet: IL-1β signaling in human TC cell lines. PTC (BCPAP and TPC1) and ATC (8505C and CAL62) cell models grown to confluence under standard conditions were analyzed by specific ELISA kits, following the manufacturer’s instructions, to evaluate ( A ) the secreted levels of IL-1β (measured in the medium) and ( B ) levels of IL1 receptor, type I (ILR1) (measured in the cell lysates), phospho-IRAK-1 (p-IRAK1) (a cell-based method), phospho-TAK1 (p-TAK1) (measured in the cell lysates), phospho-IKK (p-IKK) (a cell-based method), and P65 NF-kB (measured in the nuclear extracts). The histograms indicate mean ± SD of three different cultures, and each was tested in triplicate. The inserts represent the original histograms without ATC cells in order to better appreciate changes in PTC cells. ** p < 0.01, *** p < 0.001, and ° p < 0.001.

Article Snippet: R5010), aminoguanidine bicarbonate (AG) (cat. 396494), IL1 receptor antagonist (cat. SRP3327), and methylglyoxal (MG) (cat. M0252) were purchased from Merck Spa (Milan, Italy).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Cells

Article Title: Methylglyoxal Acts as a Tumor-Promoting Factor in Anaplastic Thyroid Cancer

doi: 10.3390/cells8060547

Figure Lengend Snippet: Glo1 depletion and related downstream events are under the partial control of IL-1β in CAL62 cells. Effects of IL-1β on ( A ) Glo1 enzyme activity; ( B ) Glo1 transcript and protein levels; ( C ) intracellular levels of MG-H1; ( D ) TGF-β1 and p-FAK levels, measured in the culture supernatant or lysate of CAL62, respectively; ( E ) migration and invasion capabilities; and ( F ) IL-1β signaling, evaluated by the levels of IL1 receptor type I (ILR1) (measured in the cell lysates), phospho-TAK1 (p-TAK1) (measured in the cell lysates), and P65 NF-kB (measured in the nuclear extracts). Western blots are representative of three different cultures, each tested in triplicate. β-actin was used as internal loading control for WB normalization. Histograms indicate mean ± SD of three different cultures, each tested in triplicate. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to untreated cells.

Article Snippet: R5010), aminoguanidine bicarbonate (AG) (cat. 396494), IL1 receptor antagonist (cat. SRP3327), and methylglyoxal (MG) (cat. M0252) were purchased from Merck Spa (Milan, Italy).

Techniques: Activity Assay, Migration, Western Blot